chef dr iii pfge system  (Bio-Rad)

Journal: Nucleic Acids Research

Article Title: Recombinase-independent chromosomal rearrangements between dispersed inverted repeats in Saccharomyces cerevisiae meiosis

doi: 10.1093/nar/gkad650

Figure Lengend Snippet: Ectopic recombination between HIS4::LEU2 and leu2::hisG creates acentric and dicentric molecules. Southern blots of chromosome III separated by PFGE and probed against ( A ) GIT1 and ( C ) CHA1 identifying dicentric and acentric molecules, respectively. ( B , D ) Quantification of ectopic recombinant signal and DSBs from panels (A) and (C) and two additional repeats (blots not shown). Error bars plotting standard deviation are shown.

Article Snippet: Chromosomes were separated using a CHEF-DR III pulsed-field gel electrophoresis (PFGE) system (Bio-Rad) using the following conditions: 1% agarose in 0.5× TBE; 14°C; 6 V/cm; switch angle 120°.

Techniques: Recombinant, Standard Deviation

Journal: Nucleic Acids Research

Article Title: Recombinase-independent chromosomal rearrangements between dispersed inverted repeats in Saccharomyces cerevisiae meiosis

doi: 10.1093/nar/gkad650

Figure Lengend Snippet: Inter-homologue/inter-sister acentric recombinant ratio is altered in rad24Δ and sgs1-md mutants. ( A ) Schematic of wild-type chromosome III and chromosome III increased in size due to insertion of pMN164 at the PRD1 locus. Inter-sister repair can lead to the formation of an acentric recombinant migrating at ∼174 kb (i) and ∼160 kb (iii) compared with an acentric recombinant forming by inter-homologue recombination of ∼167 kb (ii). ( B ) Southern blot showing chromosome III separated by PFGE and probed against CHA1 . Acentric recombinants from inter-homologue and inter-sister repair are indicated with arrows. All strains are heterozygous for PRD1 and PRD1::pMN164 . ( C ) Quantification of acentric recombinants in mutant backgrounds from panel (B) and two additional repeats (blots not shown). Error bars plotting standard deviation are shown. ( D ) Ratio of inter-homologue to inter-sister acentric recombinant formation.

Article Snippet: Chromosomes were separated using a CHEF-DR III pulsed-field gel electrophoresis (PFGE) system (Bio-Rad) using the following conditions: 1% agarose in 0.5× TBE; 14°C; 6 V/cm; switch angle 120°.

Techniques: Recombinant, Southern Blot, Mutagenesis, Standard Deviation

Journal: PLoS ONE

Article Title: Deinococcus geothermalis : The Pool of Extreme Radiation Resistance Genes Shrinks

doi: 10.1371/journal.pone.0000955

Figure Lengend Snippet: B, UV (254 nm) (3 J/m 2 s −1 ). Open circle, D. radiodurans (32°C); open triangle, D. geothermalis (50°C); and open square, D. geothermalis (32°C). Values are from three independent trials with standard deviations shown. At near-optimal growth temperatures, the 10% survival values (D 10 ) following IR for D. radiodurans (32°C) and D. geothermalis (50°C) are 15 kGy; for E. coli , 0.7 kGy (37°C) ; and for T. thermophilus (HB27) 0.8 kGy (65°C) . C, PFGE of genomic DNA prepared from irradiated (0.2 kGy) D. radiodurans (DR+IR) and D. geothermalis (DG+IR); and genomic DNA from non-irradiated D. geothermalis digested with Spe I (DG+ Spe I). (M) PFGE DNA size markers. PFGE was as described previously .

Article Snippet: The end-repaired DNA was run on a 1% TBE low melting point agarose gel for 13 hours using the following conditions (Temperature: 14°C, Voltage: 4.5 V/cm, Pulse initial: 1.0–final: 7.0 sec, Angle: 120°) on a BioRad Chef-DR III™ System PFGE system.

Techniques: Irradiation